Dr.Pallav Kaushik
Teaching Experience
• Department of Pharmacy Barkatullah University from March 2007- April 20012 For B.pharma, and
M.pharma Subjects taken- Pharmaceutical microbiology, Pharmaceutical Biochemistry,
Pharmaceutical Biotechnology & Pharmaceutical Plant biotechnology
• MSc Biochemistry from March 2007 – January 2009. Subject Cell and
Molecular Biology, Chemistry of Biomolecules, General Physiology, Microbial Genetics and
Immunology, Concepts, and application of Biotechnology.
• Department of Biotechnology from October 2011 June 2015. Cell and Molecular Biology,
Chemistry of Biomolecules, General Physiology, Microbial Genetics and Immunology, Concepts
, and the application of Biotechnology.
- City : Bhopal
- State : Madhya Pradesh
- Email ID : kaushik.pallav@gmail.com
- Profession : Scientist
- Education : Post doctorate in Biotechnology
- category : Teaching & Research
Teaching Experience
• Department of Pharmacy Barkatullah University from March 2007- April 20012 For B.pharma, and
M.pharma Subjects taken- Pharmaceutical microbiology, Pharmaceutical Biochemistry,
Pharmaceutical Biotechnology & Pharmaceutical Plant biotechnology
• MSc Biochemistry from March 2007 – January 2009. Subject Cell and
Molecular Biology, Chemistry of Biomolecules, General Physiology, Microbial Genetics and
Immunology, Concepts, and application of Biotechnology.
• Department of Biotechnology from October 2011 June 2015. Cell and Molecular Biology,
Chemistry of Biomolecules, General Physiology, Microbial Genetics and Immunology, Concepts
, and the application of Biotechnology.
- Research Experience:
Patent : 202021001557 Title: ANTI MALARIAL MOLECULES OF NATURAL
AND SYNTHETIC ORIGIN WITH DRUG CARRIER FOR PREGNANT SUBJECT.
- Women Scientist under UGC from June 2016 working in Department of Biotechnology
,Barkatullah University,Bhopal.
Tittle of project : Neurogenerative evaluation of Celastrus peniculatus and Bacopa monnieri
through drug delivery system on cultured hippocampal primordial cells subjected to
neurotoxin . - Ph.D : Ph.D in Biotechnology, under the guidance of Dr.Ragini Gothalwal, Deptt. Biotechnology
and co-guidance of Dr.A.K Pathak, Deptt. Pharmacy, B.U Bhopal.
Title of Thesis: Development of Noval Drug Delivery System for Antimal Drugs From
Natural and Synthetic Origin. - Studies Undertaken:
• Biochemical Analysis of Major Components of Synovial Joints.
• Basic Knowledge of Animal Tissue Culture.
• Cultured Chondrocytes Cells in Alginate Beads.
Training Experience:
Name of the Industry: M\S Genni, Bangalore
Techniques Learnt:
• Basic Molecular Biology Techniques.
• Plasmid Isolation.
• Agarose Gel Electrophoresis.
• DNA Isolation.
• PCR
• Rt-PCR.
Biotech. Consortium India Limited (BCIL) Sponsored Six Months Training, at Dabur Research
Foundation.
Under the guidance of Dr.Manish Diwan, Manager, Experimental Oncology Department.
IN VITRO Techniques.
A. Basic Cell Culture Techniques:
• Handling
• Maintaining the Culture.
• Sub-Culturing\ passage.
• Harvesting
• Reducing
• Freeze Down Preparation \Cryopreservation
Types of Cell Line Handled.
Primary Cultures:
I.Primary Culture of Dentritic Cells: Primary cultures of stem cells isolated from Bone marrow
preparation are maintained and studied their propagation into dentritic cells.
II.From Spleenocytes preparation: Purification and propagation of T-cell.
Secondary Culture:
Cancerous cell lines: Handling and maintenances of COLO and MIAPPACA cell line.
B. FAC`S: Harvesting, Cell staining with Fluorescence Labeled Monoclonal Antibodies:
Primary dendritic cells were treated with various phytochemicals, peptides, compounds of marine
origin for the screening of their potential as the Immunomodulatory and Anti- inflammatory
properties.
• Some cells were harvested from the primary culture which is not treated with any chemical
compound, stained with fluorescent dye attached to monoclonal antibodies; cells were then subjected
to Flow Cytometry (fluorescence activated cell sorter) which analyses large population of cell for the
amount of receptor on their surface as the level of fluorescence.
• Some cells from the primary culture were subjected to various chemical treatments
periodically, then stained with fluorescent dye attached to monoclonal antibodies and then subjected
to Flow Cytometry.
• Preliminary known to data acquisition and data analysis using Cell Quest software.
• Machine Used: FAC`S calibrator with four filters.
C. Enzyme Linked Immunosorbent Assay (ELISA)
• Used commercially available kits for detecting cytokines examples, IL-6, IL-10, IL-12,
RANTES, IFN-γ, TGF-β, and TNF-α .
• These cytokines were secreted by cell population when exposed to various chemical
compounds.
• Through the analysis of results obtained from Elisa, it is calculated which chemical is
responsible for the secretion of which cytokine, in this way surface reactor which facilitates the
secretion can be traced.
• Reference curves generated with linear regression curves fitting with r2 value >0.98.
• Hands on experience of using Graph pad prism for data analyses and interpretation of result.
D. Cytotoxic Assay (MTT Assay): MTT is yellow water soluble tetrazolium dye that is reduced by live
cells to a purple formazen product that is insoluble in aqueous solution. This assay is performed to
study the cytotoxicity of the drugs.
IN VIVO Techniques:
A. Identification and handling of small experimental animals like Rat and Mice.
B. Administration of Drugs / cancerous cell lines in experimental animals through various routes
of administration.
• Sub-cutaneous
• Intra-veneous
• Intra-muscular
• Intra peritoneal
• Oral
Hands on experience in Sub-cutaneous and Intra-veneous route of administration.
C. Dissection of animal to remove various vital and desired organs for In vitro culturing.
• Spleen for spleenocytes preparation for T-cell purification and proliferation.
• Femur bone for stem cell preparation for dentritic cells propagation.
• Lymph nodes for the study of migration of fluorescence labeled cells injected through
Sub-cutaneous route.
D. Necropsy performed on experimental animals which includes following steps;
• Exsanguination of animal.
• Gross examination of internal anatomy of animal.
• Collection and preservation of various organs and glands for pathological examination.
E. Blood with-drawl by capillary method for hematological analysis of experimental
animals depending upon their body weight.
F. Migration assay of C.F.S.E labeled T-cells in lymph nodes of lived mice.
G. Study of various type tumor in nude mouse, migration of malignant cells in a living model.
Laboratory Skills: Various other techniques learned .
• Agarose Gel Electrophoresis.
• PAGE
• Thin layer chromatography.
• Basic microbiological assays.
• Molecular biological techniques.
• Biochemical analysis of various biological compounds (viz. DNA, RNA, protein
carbohydrates).
• BOD and COD estimation.
• Isolation of DNA ,RNA & Proteins form animal tissue.
• Basic plant tissue culture.
• Animal tissue culture techniques.
Computer Knowledge: Completed a certificate course.
• Microsoft office
• Microsoft coral draw
• Adobe Photoshop
• Graph pad prism.
REVIEWER IN - Bioinfo publication
- Premier Publishers
- International Journal of agricultural sciences
- International Journal of Biotechnology
- International Journal of tropical disease and health
Registered Resource person in SREJAN.
Successfully evaluated the various scientific models and projects for 2017-18-19 and 2020.
Oral Presentations
Pallav Kaushik, R.K.Saxena,Effect of Moderate Load on Biochemical Properties of Hyaline Articular
Cartilage of Amputated Bovine Synovial Joints. National Conference on Biomechanics. Organized
by Indian Institute of Technology, New Delhi. , held on November 19th-21 th2004.
Pallav Kaushik, Anumap Pathak, Ragini Gothalwal,Effect of drug delivery systems on crude
extracts ofArtimisia annua and synthetic derivatives of artimisia on Plasmodium bhergi in vivo
female pregnant mice.National conference on new frontier for women in science and technology held
in Gwalior.(march 20-21,2013)
Pallav Kaushik Deshpande, Ragini Gothalwal, Anupam Kumar Pathak. Efficiency of nanoparticle as
a drug delivery system for natural and synthetic drugs against cerebral malaria .National Conference
on “RECENT TRENDS IN APPLIED MICROBIOLOGY, HUMAN HEALTH & ENVIRONMENT
held on 27-28,March,2015 Bundelkhand Unioversity,Jhansi.
Pallav Kaushik Deshpande, Ragini Gothalwal, Anupam Kumar Pathak .Efficiency of Liposomal drug
delivery system against Plasmodium in vitro and in vivo culture. International Conference (ECBHE2014) and 8th Annual Convention of Association of Biotechnology and Pharmacy held on 18-20
December, 2014.
Pallav Kaushik Deshpande, Ragini Gothalwal . Evaluation of Hepatoprotective action of
Andrographis paniculata In vitro cultured Hepatocytes. 2nd International Conference on Interdisciplinary Research in Engineering Management, Pharmacy and Science (ICIREMPS) -2015.
Pallav Kaushik Deshpande, Ragini Gothalwal . Green Technology: A Novel and Potential approach
for nanodrug delivery system for plant extract.National Pharma Conference .Organised by Bhabha
University and PRISAL.on 26 May 2019.
Research Article Published
Pallav Kaushik Deshpande, Ragini Gothalwal and Anupam Kumar Pathak.(2014) , Evaluation for
Efficiency of Drug Delivery Systems In vitro and In vivo Plasmodium Culture using Crude Extract
of Artemisia annua and artesunate. Current Research in Pharmaceutical Sciences ; 04 (02): 64-69
SJIF-4.102/ ICV-68.92
Pallav Kaushik Deshpande, Ragini Gothalwal and Anupam Kumar Pathak.(2014) ,Phytosomes:A
noval drug delivery system for phytoconstituents.Journal of New Biological Reports .3 (3):212-220
ISSNO 2319-1104/ IF 0.546 UGC APPROVED
Pallav Kaushik Deshpande, Ragini Gothalwal and Anupam Kumar Pathak.(2014) ,Pharmacological
properties of Andrographis paniculata. International Journal of recent advances in multidiscipilinary
research .1(4):105-0110
UGC APPROVED ISSNO 2350-0743/ SJIF-3.95
Pallav Kaushik Deshpande, Ragini Gothalwal and Anupam Kumar Pathak.(2014) ,phytochemical
analysis and antimalarial activity of Azadirachta indica A juss. The Pharma Innovation Journal ;
3(9): 12-16
UGC APPROVED ISSNO 2349-8242/NAAS RATING 5.03/ICV-82.77
Adarsh Lalit,Bheem Prasad,Pallav Kaushik,N.C Sharma,(2015),physical and phychological studies
Of men exhibiting aggressive behavior.Journal of recent initiatives in science and engineering.1:59-
62.
Pallav Kaushik Deshpande, Ragini Gothalwal and Anupam Kumar Pathak.(2015)Efficiency of
liposomal drug delivery system against plasmodium in vitro and in vivo culture.International journal
of biotechnology and Biochemistry.11,(2):105-114
UGC APPROVED IF-1.211
Pallav Kaushik Deshpande, Ragini Gothalwal (2015) .Evaluation of hepatoprotective action of
Andrographis paniculata in invitro cultured hepatocytes.International journal of current
biotechnology.3(4):7-10.
ISSNO-2321-8371 IF-0.15 UGC APPROVED
Pallav Kaushik Deshpande , Ragini Gothalwal (2016). Neuronal Cells as an Ideal Model for
Neurodegenerative Diseases International. Journal of Biotech Trends and Technology:19 (1):26-33
ISSN NO 2321-8371/ IF-2.09
Pallav Kaushik Deshpande, Rakshita Saxena, Ragini Gothalwal (2018). Effect of mercury on cultured
human lymphocytes. International journal of research in technology and management. 4(1):5-7
ISSN 2454-6240/IF-0.343
Pallav Kaushik Deshpande , Ragini Gothalwal, Anupam Kumar Pathak(2018) ,Effect of
nanoparticles on Pharmacokietic and pharmacodynamic properties of antimalarial agents.
International journal of engineering sciences and research technology:7(11),56-62
ISSN: 2277-9655 Impact Factor: 5.164
Pallav Kaushik Deshpande , Ragini Gothalwal(2018). Phytochemical screening of
Celastrus peniculatus and Bacopa monnieri for neuropotective capacity. Journal of
Plant Science Research 34 (2) 153-157
Pallav Kaushik Deshpande, Navyasai Upputuri, Ragini Gothalwal, Ramchander Merugu (2018).In
Vitro dissolution studies and enhanced antimicrobial activity of some antibiotics loaded with Copper
and Silver nanoparticles in polyvinyl alcohol films. 12, 569–573
Pallav Kaushik Deshpande , Ragini Gothalwal(2018). Formulation and validation of Phytosomes and
liposomes for various plant extracts. Proceedings of the One Day National Seminar on Innovations
and Developments in Macro, Micro and Nano Material Sciences IDMMNS-2018 pp 247-250.
Pallav Kaushik Deshpande ,Shivangi Shukla, Ragini Gothalwa l(2019). Pharmacological updates
on potential phytoconstituents of genus Ziziphus. Indian Research Journal of Pharmacy
and Science:692)1870-1878.
Pallav Kaushik Deshpande , Nisha Mishra , Ragini Gothalwal.(2019) PHYTOCHEMICAL
ANALYSIS AND HEPATOPROTECTIVE SCREENING OF BUTEA MONOSPERMA (LAM)
.Asian Journal of Research in Pharmaceutical Sciences and Biotechnology. 7(1), 2019, 6-12.
Poster Presentation
Pallav Kaushik, Anumap Pathak,Ragini Gothalwal, Evaluation of Liposomes as Drug Delivery
System in Pregnant female mice for Antimalarial drugs of Plants and Synthetic Origin.Conference
on frontiers in translational oncoimmunology, organized by All Indian Institute of Medical
Sciences,Bhopal.
Pallav Kaushik Deshpande, Ragini Gothalwal Nanoparticles as colloidal drug carriers. 2nd National
Women Conference – 2015‘Women in Science – Promoting Excellence and Innovation for Future
Development’March 25-27, 2015
Pallav Kaushik Deshpande, Ragini Gothalwal PASSIVE AND ACTIVE TUMOR TARGETING OF
NANOCARRIERS FOR ANTI-CANCER DRUG DELIVERY. International Conference on
Translational Medicine in 21st Century “Stem Cell Transplantation: Current Status” in April, 11-14,
2015 at Barkatullah University, Bhopal, India.
Pallav Kaushik Deshpande, Ragini Gothalwal .An animal cell culture: Advance technology for
modern research. National Seminar on Recent Trends in Science & Technology to be held at
MPCST,Bhopal on 30-31 March 2015
Pallav Kaushik Deshpande,Rachana Akhand Giri,Anupam Kumar Pathak. Cell suspension method
to Scale up Artimisinin production from A.annua.National conference on Plant biotechnology,Bansal
college of pharmacy,Bhopal held on march 2015.
Pallav kaushik Deshpande, Anupam Kumar Pathak. Bioremediation: A novel approach to treat textile
efflutent.National Seminar on Recent Trends in Science & Technology to be held at MPCST,Bhopal
on 30-31 March 2015
Abstract Published
Kishor Shende, Anita Misha, Pallav Kaushik, Ragini Gothalwal, Study of GC-drift and Factors
influencing codon usage in Lactobacillus species. Proceeding of Silver Jubilee bioinformatics
Conference, organized by University of Pune, Pune. (6-8 December, 2012).
Jyoti Pallav Kaushik Deshpande, Ragini Gothalwal . Stem cell therapy in curing parkinson’s
disease.International Conference on Translational Medicine in 21st Century “Stem Cell
Transplantation: Current Status” in April, 11-14, 2015 at Barkatullah University, Bhopal, India.
Nisha Pallav Kaushik Deshpande, Ragini Gothalwal .Nanoparticles a noval approach to reduce
toxicity and increase bioavailability. International Conference on Translational Medicine in 21st
Century “Stem Cell Transplantation: Current Status” in April, 11-14, 2015 at Barkatullah University,
Bhopal, India.
Anjali Pallav Kaushik Deshpande, Ragini Gothalwal .Phytosomes: a novel approach in drug delivery.
International Conference on Translational Medicine in 21st Century “Stem Cell Transplantation:
Current Status” in April, 11-14, 2015 at Barkatullah University, Bhopal, India.
Pallav Kaushik, Anumap Pathak,Ragini Gothalwal,Effect of drug delivery systems on crude extracts
of Artimisia annua and synthetic derivatives of artimisia on Plasmodium bhergi in vivo female
pregnant mice.National conference on new frontier for women in science and technology held in
Gwalior.(march 20-21,2013)
Pallav Kaushik, Anumap Pathak,Ragini Gothalwal, Evaluation of Liposomes as Drug Delivery
System in Pregnant female mice for Antimalarial drugs of Plants and Synthetic Origin.Conference
on frontiers in translational oncoimmunology, organized by All Indian Institute of Medical
Sciences,Bhopal.
Patent : 202021001557 Title: ANTI MALARIAL MOLECULES OF NATURAL
AND SYNTHETIC ORIGIN WITH DRUG CARRIER FOR PREGNANT SUBJECT.
Women Scientist under UGC from June 2016 working in Department of Biotechnology
,Barkatullah University,Bhopal.
Best Reviewer award from International journal of tropical disease and health for March 2020 and June2020.
Certificate of appreciation as My NEP Ambassador by Vidya Bharti for National Education Policy in October 2020.
